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Optical imaging, especially fluorescent microscopy, is one of the most valuable tools for studying mitochondrial bioenergetics because it provides semiquantitative and quantitative readouts as well as spatiotemporal resolution of mitochondrial metabolism

When activated, MC1R shuts down the inflammation signaling pathway called NF-kB and lowers pro-inflammatory cytokines like TNF-alpha and IL-6

Pasternak, A

subsequently, through gene function enrichment analysis (such as Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis), clarifying the regulatory roles of target genes in biological processes, molecular functions and signaling pathways