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However, this GSH-N-ethylmaleimide derivatization logistically was not possible for us as it is required during the sample collection, and we used sedimented erythrocytes provided to us from another study
Elimination : Only 3-22% is excreted unchanged in the urine (~30% of clearance occurs in the kidneys)
doi: 10.1016/j.freeradbiomed.2004.05.033
Coupled steady-state kinetic measurements with GSSR and GSH revealed that both half-reactions usually occur with second order rate constants around 10 5 to 10 6 M 1 s 1 with the reductive half-reaction being slower for wild-type glutaredoxins 11,14,15,17,19
