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Proteins were separated using SDS-PAGE and transferred to PVDF membrane (MilliporeSigma)

The cells were subsequently washed with ultrapure water and dehydrated through an anhydrous ethanol gradient

Xu L, Tao X, Gao Y, Zhang W, Meng Y, Li C, Jiang M, Xixiang Y (2017) Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L
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