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Description
To measure the activity of LnaB using ATP-- 32 P, 1 g His 6 -LnaB, 1 g Actin (Cytoskeleton, cat# APHL99) and 1.5 g PR-Ub were incubated in a 20 L reaction system containing 50 mM Tris-HCl (pH 7.5), 5 mM MgCl 2 and 5 Ci ATP-- 32 P (Perkin Elmer, cat# BLU003H250UC) for 1 h at 37 C
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184 McKemyD
In IDH1 mutant tumors, 2-hydroxyglutarate (2-HG) accumulation leads to redox imbalance
