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Honestly, they work in separate lanes

Kryiacou NM et al

Fractions were analyzed by SDS PAGE and those identified to contain tag-free GRXs were combined and subsequently spin-concentrated with a VivaSpin MWCO 3k concentrator at 4000 rpm and 4 C

Biotin does not harm your thyroid, but it can significantly distort thyroid blood test results, making it look like your levels are higher or lower than they actually are
