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Each sample was incubated three times in parallel, and an equal volume of pre-chilled acetonitrile was added at preset reaction time points, such as 0, 5, 10, 15, 30 and 60 min

This means the long chains of protein have been broken down into shorter chains of amino acids

Weeks 1-4 involve adjustment with possible increased shedding as the medication takes effect

Masi G, Fantozzi P, Villafranca A, et al
