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I listed drugs using conceptual reasoning to make sure that you, the reader, would know all potential avenues of glutathione depletion, even if it occurred due to the suppression of cysteine, or other amino acids and cofactors necessary to synthesize glutathione intracellularly

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Afterward, the PBS, NP2, and NP4 solutions were bubbled with the flowing O 2 , where the O 2 concentration was monitored using a portable dissolved oxygen meter (Hanna S20 Instruments HI-2004-02 Edge Dissolved Oxygen Meter) for 5 min

Thereafter, the cells were immediately detected by flow cytometer