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In addition, latency to the first open-arm entry, time spent in the center, total distance traveled, the number of arm transitions, and freezing time were assessed
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Exogenous NO treatment significantly increased the content of ROS in mitochondria and apoptosis, and the content of ROS in mitochondria of neurons pretreated with NO scavenger hemoglobin (Hb) was similar to that of normal cells
Then, 250 l sample solution (580 and 5800 M in sterile water) and 2.25 ml of culture medium were added into each well in triplicates
