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Proteins in liver tissues or cell lysates were electrophoretically separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred to a nitrocellulose membrane, and blocked with skimmed milk powder and incubated at 4 C overnight with primary antibodies

Studies have shown that inhibiting PHGDH expression or activity can significantly suppress metabolic reprogramming in tumor cells, demonstrating potential anti-tumor effects

doi: 10.1038/s41392-023-01481-w, 18

Platelet activation in the presence of PVL-damaged neutrophils is prevented by -defensin inhibitors and by glutathione and resveratrol, which are both inhibitors of HOCl-modified protein-induced platelet activation