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After 24 h of incubation, the cells were treated with free RhB, RhB liposomal formulation (RhB-Lip) and RhB liposomal hydrogel formulation (RhB-Lip-Gel) at a 5.0 g/mL final Rh B concentration for 3 h

therefore, any loss of hACE2 decreases insulin secretion and impairs the glucose tolerance 305,306

In addition, fasting lowers free radical levels and inflammation (Mattson and Arumugam, 2018
