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Iron typically binds to transferrin (TF) in a Fe 3+ form, thereby entering cells via transferrin receptor (TFRC) [43], then get reduced to Fe 2+ by Six-Transmembrane Epithelial Antigen of Prostate (STEAP3) metal reductase in endosome
The choice between cyanocobalamin and hydroxocobalamin might also depend on the neurological symptoms
A blocking buffer [10% BSA in PBS-T Buffer (0.05% Tween-20 v/v)] was added for 1 h at room temperature to minimize non-specific antibody binding
