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Peroxidase blocking was performed by incubating the tissues for 10 min with RNAscope H 2 O 2 at ambient temperature, after which antigen retrieval was performed by boiling the tissues with RNAscope Target Retrieval Buffer for 5 min

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Furthermore, while previous studies suggest that HIIT offers greater cardiovascular benefits than MICT 21 , there is limited research directly comparing their effects on vascular endothelial function