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It is built with stones and logs, and its main function was to serve as a strategic control point to protect the citadel from possible invaders
I have also included oral folate supplements, probiotics and have expanded my diet (but still excluding caffeine, alcohol and sugar)
For peripheral nerve analysis, a 5-mm-long piece of tail 15 mm distal from the tail base was collected from each sacrificed animal, fixed in 4% formaldehyde in phosphate buffer (pH 7.4), decalcinated, and impregnated with 1% osmium tetroxide for a few days
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