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received grants from Amgen, grants and personal fees from Amgen, Daiichi Sankyo, MSD, Pfizer and Sanofi/Regeneron, and personal fees from Aegerion Cerenis, Abbvie, Akcea, Algorithm, Astra Zeneca, Bayer, Boehringer Ingelheim, Cipla, Dr Reddys Laboratories, Esperion, Kowa, Lilly, the Medicines Company, Novartis, Silence Therapeutics, Takeda and Zuelling Pharma

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Discard if solution changes colour or becomes cloudy