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Metformin and lifestyle modification in polycystic ovary syndrome: systematic review and meta-analysis

His-C16orf87 and GST-HDAC1 protein purification Plasmids encoding different 8 His-C16orf87, GST, and GST-HDAC1 proteins were transformed into BL21(DE3) competent cells, bacterial cultures were grown to OD 600 = 0.5, and protein expression was induced with 1 mM IPTG at 37 C for 3 h