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For Caspase-3 staining, the tissues were blocked with 10% normal horse serum for 30 min then incubated with Caspase-3 antibody (1:300, BD Biosciences, Macquarie Park, NSW, Australia) overnight, followed by secondary antibodies (Goat anti-rabbit HRP, 1:200, Vector Laboratories, Burlingame, CA, USA) for 45 min

Metabolic changes induced by maximal exercise in human subjects following L-carnitine administration

To examine the potential contribution of gut microbiota to TMAO formation from dietary L-carnitine, volunteers were placed on oral poorly absorbed broad spectrum antibiotics to suppress intestinal microbiota for a week, and then repeat L-carnitine challenge performed (Visit 2)

Depende del tipo y de las expectativas