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The 1984 NIH Consensus Conference CHRIS MASTERJOHN: So if you go back to 1984, when we saw that Time magazine picture, the other thing that was going on politically and scientifically in 1984 was the NIH Consensus Conference that said that they had proven, like Time magazine cover was a reaction to the NIH consensus conference where they said, We as the scientific community now certify that we are in consensus that cholesterol is the cause of heart disease. And thats why Time magazine ran with that cover

For the amplicon PCR, a 25 L reaction containing cDNA, 0.5 L of Platinum SuperFi II DNA Polymerase, 1 SuperFi II Buffer, and 500 nM forward and reverse primers was first heated to 98 C for 30 s, followed by cycles of 98 C for 10 s, 64 C for 10 s, and 72 C for 20 s

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