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Description
An integrated metabolomics workflow for the quantification of sulfur pathway intermediates employing thiol protection with N-ethyl maleimide and hydrophilic interaction liquid chromatography tandem mass spectrometry
Cells were collected, pelleted and washed once with ice-cold 1 PBS, followed by two washes with 1 PBS + 50 mM iodoacetamine (IAA) (BioRad) with 5 min of rocking at RT for the second wash
Gastroenterology 141, 599609
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