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Glutathione is the essential cofactor for many enzymes which require thiol-reducing equivalents, and helps keep redox-sensitive active sites on enzymes in the necessary reduced state

Post-induction the cells were pelleted down at 6,000 x g for 15 min at 4C and the pellets were resuspended in 4XT (200 mM Tris, pH 8.0, 800 mM NaCl, 1 mM DTT) supplemented with protease inhibitors (1 mM AEBSF, 0.8 M Aprotinin, 15 M E-64, 10 g/mL Bestatin, 10 g/mL Pepstatin A and 10 g/mL Leupeptin) and lysed by sonication

If you experience persistent pain, spreading redness, warmth, or discharge from an injection site, seek medical attention

NADPH is used as a cofactor by Glutathione Reductase to reduce oxidized glutathione (GSSG2GSH), and likewise by thioredoxin reductase to reduce oxidized thioredoxin