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Centrifuge at 10,000 x g for 2 minutes (or sufficient to produce a clear supernatant) Collect the supernatant and aliquot an appropriate portion for protein analysis

doi:10.1016/S0968-0004(03)00114-2

Patients were treated for 80 days with ALA (600 mg/day, treatment group) or with a placebo (control group)

As one of the main components, crocin improved depressive symptoms and reduced the expression of inflammatory cytokines (e.g., IL-1, IL-18, and TNF-) in the hippocampus of LPS-depressed mice (96)
