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B Vitamins are water-soluble and excess is excreted through the urine

They were cultured, respectively in Dulbecco's Modified Eagle Medium, Iscove's Modified Dulbecco's Medium, and Roswell Park Memorial Institute 1640 medium (Hyclone) with 10% fetal bovine serum (Gibco) under incubation at 37C with 5% carbon dioxide

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