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Then the tumorspheres were collected and transferred to 48-well plates (1 tumorsphere per well) and exposed to different drugs (PBS, PS-CHO + L, ATRA-SS-ATRA, PS-CHO@ATRA-SS-ATRA, PS-CHO@DSPE-PEG2000 + L and PS-CHO@ATRA-SS-ATRA + L) at the concentration of 20 g/mL

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Autologous fibroblasts induce fibrosis of the nucleus pulposus to maintain the stability of degenerative intervertebral discs