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Molecular drivers and cortical spread of lateral entorhinal cortex dysfunction in preclinical Alzheimers disease

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Then, heart tissue was homogenized mechanically in icecold homogenization buffer (50 mmol/L TrisHCl (pH 7.4), 3.1 mmol/L sucrose, 1 mmol/L dithiothreitol, 10 mg/mL, leupeptin, 10 mg/mL soybean trypsin inhibitor, 2 mg/mL aprotinin and 0.1% Triton X100) (SigmaAldrich, Munich, Germany)

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