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An adequate volume of each cell culture was inoculated into 20 mL of the same medium in 200 mL of baffled erlenmeyer flask with or without 300 mg/L l-Ser to achieve an initial OD 600 value of 0.15

Injectable and IV Glutathione: Off-Label Use vs Unapproved Drug Injectable glutathione is not FDA-approved as a drug product, which means it cannot be marketed, sold, or administered as a treatment for any medical condition without meeting the criteria for lawful off-label prescribing

This alternative hypothesis proposes arrestin-driven receptor-G protein uncoupling (desensitization) as the mechanism responsible for the loss of morphine's analgesic action, which is in turn exacerbated by receptor failure to internalize and undergo resensitization [38, 39]

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