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During the validation and optimization method, the internal standards dissolved in the four solvents were tested in a mixture of 100 % methanol, methanol/water (50:50) (v/v), methanol + 0.01 % acetic acid, and ammonium buffer pH 3.5 [H 2 O/ACN (90:10) (v/v) + 1 mM NH 4 (CH 3 COO)]

Although studies show that the mutations in those genes modulate the risk to develop neuropathy following vincristine to some degree, additional sufficiently powered studies may be required to validate the association between these genetic polymorphisms and pediatric peripheral neuropathy

Results appear over multiple sessions under medical care