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Lavar con 3 mL de solucin acuosa de cido clorhdrico 10 mmol/L, desechar el lavado, secar al vaco durante 1 min, eluir con 2 mL de solucin de amonaco al 4% en metanol, recolectar el eluato en un tubo de centrifugacin de 15 mL, evaporar a sequedad bajo corriente de nitrgeno a 40C, el residuo obtenido se reserva para la derivatizacin

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