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This trend of increasing mortality with both NP concentration and exposure duration indicated that while low doses of MnO 2 NPs were largely tolerated, higher doses gradually exceeded the embryos capacity to cope, leading to death in a fraction of the population

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Ethics statement The animal study was reviewed and approved by Shanghai University of Traditional Chinese Medicine Animal Experiment Ethics Committee

Letting the sample evolve, after 6 h, the main species in the solution are oligomeric or pre-fibrillar species