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Notably, the NPC1 -knockout cells yielded the majority of both sensitizing and desensitizing hits (65% and 77%, respectively), consistent with the damage-prone state of this genetic background

Bibcode:2003Sci...301..361K

doi: 10.1111/jcmm.15318 29 LiWLiWLengYXiongYXiaZ
Phage display libraries are indisputably useful especially for epitope mapping, vaccine development, bioactive peptides and some non-peptide structures and the ligands determined using this method are appropriate for chromatographic analyses, nevertheless there are some cases that limit the use of this technique, such as some optimization problems and some issues as working in large scale as well as the limitation of the application since peptides does not work unless it is a part of the phage, not in free solution [2]
