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In addition, when comparing the metabolites in the urea cycle, we found that the change of arginine and citrulline in the egg yolk was almost coordinated with each other, which might support that the yolk-metabolized arginine was involved in the process of ammonia metabolism (Figure 4A)

The improvement in reproductive antioxidant defense systems may be due to the increase in reduced glutathione concentration and activities of glutathione peroxidase, catalase, and SOD enzymes (28, 61, 62), which protect the reproductive organs' defense systems against ROS attacks

Ethical statement The experimental design and reporting followed the ARRIVE guidelines to ensure transparency and reproducibility