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Description
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For the construction of the metE mutant, the AES -which contained a Kan R cassette and the GFP gene optimized for its expression in mycobacteria (eGFP) (Supplementary Note 1)- was synthesized by GenScript
Subsequently, the nanomicelles were loaded into a carbopol 934based hydrogel with eucalyptus oil to prepare an MTX nanomicelleloaded hydrogel
Protein detection was accomplished using an enhanced BCA protein assay kit (Byeotime, Shanghai, China)
