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Description
IKK2-EE
Normal rabbit IgG (#I8410, Sigma) was used as a non-specific antibody control
The Grx1-roGFP2 fusion protein, which combines human glutaredoxin with roGFP2, further enhances sensitivity and dynamic range, making it particularly effective for monitoring rapid and subtle changes in glutathione redox potential across different cellular compartments
Captured fragments were retrieved with streptavidin-coated beads, washed, and enriched by post-capture PCR
