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pcDNA3.1-3xFlag-Grx1 was made by inserting Grx1 cDNA into the pcDNA3.1-3xFlag vector using BamHI and XhoI sites

glutamicum ( CgMCS gene) or Escherichia coli ( EcMCS gene) in NG13, introduced by pCMCS or pEMCS plasmids, respectively, provided similar glutamate production levels to that with the CgCS strain (Supplementary Fig

We've observed measurement errors compound across multi-vial protocolsif a researcher under-doses by 50mcg per injection due to poor concentration planning, that's 3,000mcg (3mg) error across a 60-dose protocol, equivalent to six full missed doses

Unfortunately, veterinary application, trials, and research in dogs are still 510 years behind human medicine, but interest in the field is rapidly accelerating