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Vitamin B12 Intramuscular (IM) Injections have become a popular method of supplementing this essential nutrient

Results A counter-selection system was engineered in Saccharomyces cerevisiae based on the known feedback inhibition of gamma-glutamylcysteine synthetase (GSH1) gene expression, which is rate limiting for GSH synthesis: the GSH1 ORF and the counter-selectable marker GAP1 were expressed under control of the TEF1 and GSH-regulated GSH1 promoters, respectively

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A total of 200 M hydrogen peroxide (H2O2) significantly induced cytotoxicity in HeLa cells, while H2O2 up to 300 M did not induce cytotoxicity in HepG2 cells, further demonstrating the strong resistance of HepG2 cells to ROS