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MCF-7 cell lines (ATCC, USA) were cultured in RPMI 1640 media (EuroClone S.p.A., Italy) supplemented with 1% penicillin-streptomycin, 1% L-Glutamine, and 10% fetal bovine serum (FBS) and incubated in a 37 C incubator under 5% CO2 atmosphere

These reversible posttranslational modifications (PTMs) enable redox-sensitive dynamic changes in cell signaling and function to maintain redox homeostasis in various cellular compartments, protect organisms from oxidative insults, and actively participate in redox-regulatory and signaling processes (41)