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Our TLC/GC analysis revealed that two mutant strains, tub-2 and egl-4(gf) , which had been reported have high fat stores based on Nile Red staining of live worms [26][29], actually have wild type levels of fat stores

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Organic solvents such as methanol (MeOH), ethanol (EtOH), acetonitrile (ACN), acetone, hexane, chloroform (CHCl 3 ), 2-propanol (iPrOH), acetic acid (AcOH), xylene, toluene, and tert -butyl methyl ether can remove small molecules, thereby improving in situ protein detection and imaging by reducing ion suppression from salts, lipids, and metabolites

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