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DDA mass spectra were analyzed with Proteome Discoverer (version 3.0.1.27) and searched by using Sequest HT + INFERYS rescoring against the human SwissProt database (version 2023-06-28) plus a custom KRAS G12C database with dynamic methionine oxidation and static cysteine carbamidomethyl modifications

Building on preliminary studies, this research further examines the effects of prenatal DEHP exposure on the sexual development of offspring female rats, particularly investigating whether DEHP induces precocious puberty in females via the hypothalamic ER-mediated IGF-1 and NKB signaling pathways

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