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Individual favorable mutations were combined as described in the text via subcloning using a Bam HI restriction site at the amino acid positions P317/D318 in conjunction with the Sac I restriction site

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(2) the ability to grow in three dimensions (3D) in the absence of intercellular matrix attachment is a necessary hallmark that transforming cells must acquire to develop clinical tumors 33 , and requires quenching an excess of reactive-oxygen species (ROS) 34