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Results Overall structures of CCK1R and CCK2R signaling complexes For structural studies with different transducer-coupled CCKRs (CCK1RG s and CCK2RG q ), human CCK1R and CCK2R with 15-residue depletion at the C-terminus were subcloned into pFastBac1 vector with an N-terminal Flag-tag, along with a LgBiT and double maltose-binding protein (MBP) affinity tag at the C-terminus to facilitate the protein expression and purification 42

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7.3 Inositol Inositol (also known as vitamin B8) is a carbohydrate compound that is an essential component of the plasma membrane phospholipids and has an important role in synthesizing secondary messengers in the cells (200)

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