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Samples were then homogenized via a Bioruptor bath sonicator (Diagenode, set on high power with 10 cycles of 10 s pulses with a 10 s pause between pulses, at 4C), then centrifuged at 21,500 x g at 4C for 30 min and the supernatant retained
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In a subsequent publication, Gaitan et al
After 24 h, the cells were washed with PBS, and then 500 l medium containing 10% v/v Cell Counting Kit solution was added to each well
