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Oxidative stress (OS) occurs when there is an overproduction of oxidative free radicals and ROS, which damage spermatozoa and cause male infertility by impairing both the structure and function of sperm (Aitken et al

The 2 Ct method was used to determine relative gene expression, presented as the mean standard error of 3 independent experiments

Further research is necessary to elucidate the broader implications of choline metabolism in immune regulation and inflammatory diseases

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