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Fluorescent secondary antibodies (1:200 in blocking buffer) were applied for 2 h at room temperature in the dark, followed by four 7-min PBST washes
doi: 10.1186/s12929-022-00861-8 184 FerreiraAVAlarcon-BarreraJCDomnguez-AndrsJBulutKilicGDebisarunPAet al
Both FOXO4 and TP53 bound ES2 peptide, while no binding was detected for GFP
S.HardinghamG
