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Vlacil AK, Bnfer S, Jacob R, Trippel N, Kuzu I, Schieffer B, et al

Steroid Biochem.1986, 25 (5), 867-876 72

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For ratiometric sensors, quantifying fluorescence emission in areas with poor/no signal can lead to artefactually large variation and a low signal-to-noise ratio as can including pixels where the detector is saturatedsuch areas should be removed prior to interpretation (Figure 2)
