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In addition to the chemical methods mentioned above, enzymes such as peroxygenase and other epoxy alcohol synthases/hydroperoxide isomerases can be used to generate hydroxy-epoxides from hydroperoxides
Like many peptide materials, GHK-Cu may demonstrate reduced stability in aqueous solution over time, particularly under conditions of elevated temperature or prolonged light exposure
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BCA or Bradford protein assays were performed to determine protein concentrations, and the fractions were either used fresh or stored in aliquots at -80C (cellular membrane fractions) or -20C (cytosolic fractions)
