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While the flow cytometric apoptosis reduction (3.48%) was not statistically significant, the molecular markers (caspase-3 down 42.7%, PARP down 77.5%, Bcl-2/Bax shift) were all highly significant

The cel ls were pelleted after 20 hours by centrifugation using a Fiberlite F9-61000 rotor in a Sorvall LYNX 6000 Superspeed centrifuge at 2,000 g for 20 minutes, re-suspended in 40 ml lysis buffer containing 50 mM Tris, pH 7.5, 150 mM NaCl, 20 mM imidazole, 20 % glycerol, 2 mM TCEP, 250 units of Benzonase Nuclease and bacterial Protease Inhibitor Cocktail (Amresco

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Module detection and analysis In order to get an insight on the cellular role of the network, a Cytoscape plug-in, MCODE (1.31) was used to identify these highly interconnected regions in the present protein-protein interaction network