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The assays limit of detection was below 0.5 ng/ml, enabling reliable quantification of low 8-OHdG concentrations, which were expressed in ng/ml
After freezing at 80 C and thawing once, the sample was centrifuged at 13,000 x g for 30 min at 4 C, and 100 l of supernatant was saved
Vitamin D counteracts the activity of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, which is responsible for ROS production, and enhances the antioxidant capacity by increasing the activity of antioxidative enzymes such as superoxide dismutase [146]
