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After a brief PBS rinse, the cells were trypsinized and resuspended in 500 l PBS and analyzed in an Attune NxT flow cytometer (Thermo Fisher Scientific) with the BL1 detector using the 488 nm laser line

We'll use a standard approach for a 250 mcg dosage from a 5 mg BPC-157 vial reconstituted with 2 mL of BAC water

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