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A pseudocontinuous ASL (pCASL) technique was applied using a three-dimensional single-shot gradient and spin echo (3D-GRASE) sequence with the parameters: TE of 12 ms, TR of 3772 ms, FOV of 220 220 mm, voxel size of 2.75 2.75 mm, slice thickness of 6 mm, 22 slices, a labeling duration of 1800 ms, and a post labeling delay of 1600 ms

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The instrument performed a survey MS acquisition (TOFMS) from m/z 1201,250 (250 ms accumulation time), followed by MS/MS (high sensitivity mode) from m/z 801,500 on the 15 most intense precursor ions (excluded for 20 s after two occurrences) using information dependent acquisition with dynamic background subtraction