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The total cell lysate was centrifuged at 20,000 rpm for 30 min at 4 C, and the soluble fraction was loaded onto a Ni-NTA agarose resin column (Qiagen) that was pre-equilibrated with Buffer A
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Reduced brain GABA in primary insomnia: preliminary data from 4T proton magnetic resonance spectroscopy (1H-MRS)
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A segment of mid-esophagus was excised within 60 min of circulatory arrest and preserved in PBS buffer until processing
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