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doi: 10.1016/j.jep.2024.118956 61 GaoSFengQ
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After exposure, the tissues were placed back into their multi-well culture plates containing the same medium, a further aliquot of media was added apically (20 L for Akemi, 75 L for all others except LPS) and returned to incubator until next exposure
The tissue lysate was centrifuged to remove debris, and the supernatant was collected for further analyses
