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For KGA rescue experiment, the used plasmid was previously altered to confer geneticin resistance 7 , using neo (neomycin phosphotransferase) coding sequence extracted from pQCXI Neo DsRed-LC3-GFP, a gift from David Sabatini (Addgene plasmid #31183), generating pQC MCS IRES G418 (Addgene # 110344) and pQC V5 MCS IRES G418 (Addgene # 110345)

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Many people have difficulty maintaining the diet (Vahedi 2003